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Antibody discovery using human naive scFv phage display |
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Identifying antibody leads is a truly laborious and costly process. Although there is an option to outsource certain standardized steps, taregt specific selection strategies and screening are often required to meet the specific project's needs and broader portfolio pipeline goals. To meet these specific requirements, Myxengo offers Antibody Discoveryt Service, in which our highly trained and experienced scientists will provide high-quality deliverables in a timely and cost effective manner. We deliver fully human IgG as a final resulted outcome which make us different from other service providers delivering scFv or Fab.
Due to the big size of our library and separate selection for kappa and lambda library, we have confidence to provide best antibody leads for many targets within very short amount of time. |
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STEPS |
TIMEFRAMES |
Target validation (Biotinylation, evaluation) |
1~2 weeks |
Selection of scFvs from naive human scFv library (Kappa and Lambda separately) |
2~4 weeks |
High throughput screening up to 1,000 clones |
1~2 weeks |
DNA sequencing and identifying unique clones |
1~2 weeks |
Full reformatting into IgG and transfection into mammalian cell |
1~2 weeks |
Final evaluation of the expressed IgG and shipping with sequence + DNA (up to 50 clones) |
1~2 weeks |
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© Copyright 2010 Myxengo, Inc
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